Comparison of three DNA extraction methods for polymerase chain reaction (PCR) analysis of bacterial genomic DNA
نویسندگان
چکیده
منابع مشابه
Comparison of three DNA extraction methods for polymerase chain reaction (PCR) analysis of bacterial genomic DNA
Rapid isolation of high-purity microbial genomic DNA is necessary for genome analysis. In this study, the authors compared a one-hour procedure using a microwave with enzymatic and boiling methods of genomic DNA extraction from Gram-negative and Gram-positive bacteria. High DNA concentration and purity were observed for both MRSA and ESBL strains (80.1 and 91.1 μg/ml; OD260/280, 1.82 and 1.70, ...
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ژورنال
عنوان ژورنال: African Journal of Microbiology Research
سال: 2014
ISSN: 1996-0808
DOI: 10.5897/ajmr2013.6459